Effect of congsheng capsule on intracellular calcium concentration in mice after ischemic cerebral injury / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of Congsheng Capsule (CSC) in intracellular Ca2+ concentration in cerebral intra-synaptosomes of ischemia/reperfusion mice and analogous ischemic injured neurons to estimate the mechanism of CSC in antagonizing ischemic cerebral injury.</p><p><b>METHODS</b>The concentration of Ca2+ in cerebral intra-synaptosomes of mice and in neurocyte suspension of fetal mice were determined using Fura-2/AM double wavelength fluorescence spectrophotometer.</p><p><b>RESULTS</b>CSC 3 g/kg and 9 g/kg through gastrogavage could relieve the degree of calcium overloading of intra-synaptosomes in mice after cerebral ischemia/reperfusion. The CSC containing serum could inhibit the elevation of Ca2+ content in neurocytes induced by 1 hour hypoxia and glutamate of high concentration (200 mumol/L).</p><p><b>CONCLUSION</b>CSC plays a role in antagonizing ischemic injury through relieving the calcium overloading after cerebral ischemia.</p>