Chromosomal translocation involving USP6 gene in nodular fasciitis / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 533-536, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-304458
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the frequency of USP6 gene rearrangement in nodular fasciitis (NF) and to evaluate its clinical application.</p><p><b>METHODS</b>Twenty nine cases of previously diagnosed NF were screened for the presence of the USP6 gene rearrangement by interphase fluorescence-in-situ hybridization (FISH) on formalin-fixed paraffin-embedded tissue. Fifteen of these cases, which had available tissue, were also analysed for MYH9-USP6 fusion transcripts by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Twenty four of the 29 cases (83%) were positive for the USP6 gene rearrangement by interphase FISH. The 15 cases with RT-PCR showed the following results:
11 positive, one deletion and three negative for USP6 gene rearrangement. Of these 15 cases, eight (8/15) showed MYH9-USP6 fusion transcript by RT-PCR. Of these eight cases, seven were positive for USP6 gene rearrangement and one showed USP6 deletion by FISH.</p><p><b>CONCLUSIONS</b>USP6 gene rearrangement is a recurrent genetic event in NF. It is a valuable ancillary tool for the pathological diagnosis of these lesions.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Translocación Genética
/
Reordenamiento Génico
/
Proteínas Proto-Oncogénicas
/
Hibridación Fluorescente in Situ
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Ubiquitina Tiolesterasa
/
Fascitis
/
Genética
/
Interfase
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Pathology
Año:
2014
Tipo del documento:
Artículo
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