Your browser doesn't support javascript.
loading
Functional divergence of betaine aldehyde dehydrogenase genes in Populus euphratica / 生物工程学报
Chinese Journal of Biotechnology ; (12): 329-339, 2012.
Artículo en Chino | WPRIM | ID: wpr-304489
ABSTRACT
Plant betaine aldehyde dehydrogenase (BADH) is a physiologically important enzyme in response to salt or drought stress. In this study, two BADH genes (PeBADH1 and PeBADH2) were cloned from Populus euphratica. Both PeBADH1 and PeBADH2 genes encode the proteins of 503 amino acid residues, with a calculated molecular mass of 54.93 kDa and 54.90 kDa, respectively. Reverse transcription PCR showed the divergence of expression pattern between the PeBADH1 and PeBADH2 genes in P. euphratica. The recombinant PeBADH1 and PeBADH2 proteins were overexpressed in E. coli, and purified by Ni-affinity chromatography. The PeBADH2 protein had 1.5-fold higher enzymatic activity towards the substrate aldehyde than PeBADH1 protein. The PeBADH1 protein revealed higher thermal stability than PeBADH2 protein. These results indicated obvious functional divergence between the PeBADH1 and PeBADH2 genes.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Proteínas de Plantas / Especificidad por Sustrato / Proteínas Recombinantes / Datos de Secuencia Molecular / Química / Secuencia de Aminoácidos / Clonación Molecular / Regulación de la Expresión Génica de las Plantas / Isoformas de Proteínas Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2012 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Proteínas de Plantas / Especificidad por Sustrato / Proteínas Recombinantes / Datos de Secuencia Molecular / Química / Secuencia de Aminoácidos / Clonación Molecular / Regulación de la Expresión Génica de las Plantas / Isoformas de Proteínas Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2012 Tipo del documento: Artículo