Progress in expression and molecular modification of microbial transglutaminase / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1681-1689, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-304532
ABSTRACT
Microbial transglutaminase, which could catalyze the cross-linking of many proteins or non-protein materials, has been widely used in food, pharmaceutical and textile industry. To enhance the yield of the enzyme and establish corresponding platform for molecular modification, the researchers of Japanese Ajinomoto began to construct the recombinant strain producing transglutaminase in the 1990s. So far, the enzyme has been successfully expressed in different expression systems. Some of the recombinant strains are more productive than wild strains. Recently, progress has been made in the molecular modification of microbial transglutaminase, and the activity, thermo-stability and specificity of the enzyme are improved. This review briefly summarized and analyzed the strategies involved in these studies, and noted its trends.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Streptomyces
/
Especificidad por Sustrato
/
Proteínas Bacterianas
/
Estabilidad de Enzimas
/
Ingeniería de Proteínas
/
Catálisis
/
Transglutaminasas
/
Mutagénesis Sitio-Dirigida
/
Proteínas Mutantes
/
Genética
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2011
Tipo del documento:
Artículo
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