Large-scale in vitro preparation of new gp96 tumor vaccine and analysis of its induction of specific anti-tumor immunoresponses / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1598-1605, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-304541
ABSTRACT
Heat shock protein gp96 isolated from tumor tissues holds great promise for tumor immunotherapy. However, at present only very limited amount of gp96 protein can be isolated from tumor tissues. Here, we reconstituted the yeast-expressed gp96 (recombinant gp96, rgp96) with B16.F10 melanoma antigens in vitro to prepare new gp96 tumor vaccine on large-scale, and analyzed its induction of specific anti-tumor immunoresponses by ELISPOT, IFN-gamma intracellular staining and cytotoxicity assays. Immunization with rgp96-tumor antigen complexes significantly inhibited B16 tumor growth compared with either rgp96 or tumor antigens alone and led to enhancement of tumor-specific T-cell activities, which was found similar to that of tumor tissue derived gp96. Our results therefore may provide bases for large-scale preparation of the new generation of gp96 tumor vaccines.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neoplasias Cutáneas
/
Terapéutica
/
Levaduras
/
Melanoma Experimental
/
Proteínas Recombinantes de Fusión
/
Vacunas contra el Cáncer
/
Usos Terapéuticos
/
Alergia e Inmunología
/
Genética
/
Proteínas de Choque Térmico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2011
Tipo del documento:
Artículo
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