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Correlation of the DNA fragmentation index and malformation rate of optimized sperm with embryonic development and early spontaneous abortion in IVF-ET / 中华男科学杂志
National Journal of Andrology ; (12): 520-524, 2016.
Artículo en Chino | WPRIM | ID: wpr-304707
ABSTRACT
<p><b>Objective</b>To investigate the effects of the DNA fragmentation index (DFI) and malformation rate (SMR) of optimized sperm on embryonic development and early spontaneous abortion in conventional in vitro fertilization and embryo transfer (IVF-ET).</p><p><b>METHODS</b>We selected 602 cycles of conventional IVF-ET for pure oviductal infertility that had achieved clinical pregnancies, including 505 cycles with ongoing pregnancy and 97 cycles with early spontaneous abortion. On the day of ovum retrieval, we examined the DNA integrity and morphology of the rest of the optimized sperm using the SCD and Diff-Quik methods, established the joint predictor (JP) by logistic equation, and assessed the value of DFI and SMR in predicting early spontaneous abortion using the ROC curve.</p><p><b>RESULTS</b>The DFI, SMR, and high-quality embryo rate were (15.91±3.69)%, (82.85±10.24)%, and 46.53% (342/735) in the early spontaneous abortion group and (9.30±4.22)%, (77.32±9.19)%, and 56.43% (2263/4010) respectively in the ongoing pregnancy group, all with statistically significant differences between the two groups (P<0.05 ). Both the DFI and SMR were the risk factors of early spontaneous abortion (OR = 5.96 and 1.66; both P< 0.01). The areas under the ROC curve for DFI, SMR and JP were 0.893±0.019, 0.685±0.028, and 0.898±0.018, respectively. According to the Youden index, the optimal cut-off values of the DFI and SMR obtained for the prediction of early spontaneous abortion were approximately 15% and 80%. The DFI was correlated positively with SMR (r= 0.31, P<0.01) but the high-quality embryo rate negatively with both the DFI (r= -0.45, P<0.01) and SMR (r= -0.22, P<0.01).</p><p><b>CONCLUSIONS</b>The DFI and SMR of optimized sperm are closely associated with embryonic development in IVF. The DFI has a certain value for predicting early spontaneous abortion with a threshold of approximately 15%, but SMR may have a lower predictive value.</p>
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Espermatozoides / Fertilización In Vitro / Aborto Espontáneo / Factores de Riesgo / Curva ROC / Desarrollo Embrionario / Transferencia de Embrión / Fragmentación del ADN / Infertilidad Tipo de estudio: Estudio de etiología / Estudio pronóstico / Factores de riesgo Límite: Femenino / Humanos / Masculino / Embarazo Idioma: Chino Revista: National Journal of Andrology Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Espermatozoides / Fertilización In Vitro / Aborto Espontáneo / Factores de Riesgo / Curva ROC / Desarrollo Embrionario / Transferencia de Embrión / Fragmentación del ADN / Infertilidad Tipo de estudio: Estudio de etiología / Estudio pronóstico / Factores de riesgo Límite: Femenino / Humanos / Masculino / Embarazo Idioma: Chino Revista: National Journal of Andrology Año: 2016 Tipo del documento: Artículo