Study on antiplatelet and antithrombin activitives and effective components variation of Puhuang-Wulingzhi before and after compatibility / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3187-3193, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-304835
ABSTRACT
The changes of bioactive constituents were analyzed for Puhuang-Wulingzhi before and after compatibility and the antiplatelet and antithrombin activitives were evaluated in order to elucidate the scientific and reasonable of Puhuang-Wulingzhi compatibility. UPLC-QTOF-MA-Markerlynx, principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis were used for data analysis and tracking changes of chemical composition during the decocting process. In vitro platelet aggregation induced by ADP, thrombin time(TT) and prothrombin time (PT) were investigated for Puhuang-Wulingzhi before and after compatibility. The results showed that significant differences were found between the mixed decoction and codecoction of Wulingzhi and Puhuang. Five compounds changed obviously were identified as typhaneoside, naringenin, isorhamnetin-3-O-ruinoside, quercetin-3-O-neohesperidoside, kaempferol-3-O-neohesperidoside. The codecoction, comparing with the single decoction, was more significant in antiplatelet aggregation and could prolong thrombin time. In the same crude drug dose, the thrombin time (TT) elongation were greater. These data could provide references for elucidation of bioactive components for this herb pair.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fisiología
/
Tiempo de Trombina
/
Plaquetas
/
Medicamentos Herbarios Chinos
/
Estructura Molecular
/
Química
/
Agregación Plaquetaria
/
Antitrombinas
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2015
Tipo del documento:
Artículo
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