Identification of Mycobacterium Species by Multiplex PCR-Restriction Fragment Length Polymorphism Assay / 감염
Korean Journal of Infectious Diseases
;
: 148-152, 1999.
Artículo
en Coreano
| WPRIM
| ID: wpr-30485
ABSTRACT
BACKGROUND:
Recently the clinical significance of several mycobacterial species has been increased and there is a growing need to identify mycobacteria to the species level. We evaluated multiplex polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) assay for identification of mycobacterial isolates.METHODS:
Reference strains of 6 species of mycobacteria and 88 clinical isolates were lysed by boiling method. The lysates were used for multiplex PCR reactions incorporating three pairs of PCR primers, which were expected to amplify fragments from the 65-kDa gene common to all mycobacteria, genes of M. tuberculosis complex and M. avium, respectively. The resultant amplicons were digested with the restric-tion enzymes PspEI and HaeIII. Multiplex PCR products and digested products were visualized by electrophoresis on agarose gels.RESULTS:
Six reference strains yielded compatible results. Eighty-eight clinical isolates were identified as M. tuberculosis complex (81 strains), M. avium (2 strains), M. intracellulare (2 strains), M. fortuitum biovariant peregrinum (2 strains), and M. gordonae III (1 strain).CONCLUSION:
Multiplex PCR-RFLP assay appears to be a reliable method for rapid identification of mycobacteria to species level.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sefarosa
/
Tuberculosis
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Reacción en Cadena de la Polimerasa
/
Bacteria Gordonia
/
Electroforesis
/
Reacción en Cadena de la Polimerasa Multiplex
/
Geles
/
Mycobacterium
Tipo de estudio:
Estudio diagnóstico
Idioma:
Coreano
Revista:
Korean Journal of Infectious Diseases
Año:
1999
Tipo del documento:
Artículo
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