Autologous bone marrow mononuclear cells and peripheral endothelial progenitor cells differentiation in myocardial ischemia reperfusion injury region in swine / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 350-353, 2007.
Article
en Zh
| WPRIM
| ID: wpr-304906
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differentiation status of autologous bone marrow mononuclear cells (BM-MNC) and peripheral endothelial progenitor cells (EPC) transplanted into myocardial ischemia reperfusion injury region in swine.</p><p><b>METHODS</b>BM-MNC marked with PKH26 (n = 9), EPC marked with CM-DiI (n = 7), phosphate buffer saline (control, n = 7) were transplanted into myocardial ischemia reperfusion injury region of swine by intracoronary artery injection. Specimens were harvested 4 weeks after injection for histological analysis (HE, immunochemical stain for vWF, alpha-sarcomeric-actin and fibronectin antibody). Cell differentiation was observed under transmission electronmicroscope.</p><p><b>RESULTS</b>The number of small blood vessels was similar between BM-MNC group and EPC group (13.39 +/- 6.96/HP vs.12.39 +/- 4.72/HP, P < 0.05), but was significantly higher than that of control group (P < 0.05). Responsive intensity of immunochemical stain for fibronectin antibody was significantly lower in BM-MNC and EPC groups than that in control group. Responsive intensity of immunochemical stain for alpha-sarcomeric-actin antibody was similar among the three groups. Cluster cells were observed in one swine from BM-MNC group which might relate to the proliferation of stem cells in situ. Immature endothelial cells and myocytes were also detected by transmission electronmicroscope in BM-MNC and EPC group.</p><p><b>CONCLUSION</b>BM-MNC and EPC transplanted into myocardial ischemia reperfusion injury region in swine stimulated the formation of blood vessels and inhibited fibrogenesis.</p>
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Índice:
WPRIM
Asunto principal:
Células Madre
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Porcinos
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Porcinos Enanos
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Trasplante
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Trasplante Autólogo
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Sangre
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Células de la Médula Ósea
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Monocitos
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Daño por Reperfusión Miocárdica
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Diferenciación Celular
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Cardiology
Año:
2007
Tipo del documento:
Article