Noninvasive detection of experimental vulnerable atherosclerotic plaques with 99Tc m labeled C2A domain of synaptotagmin I / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 178-181, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-304943
ABSTRACT
<p><b>OBJECTIVE</b>(99)Tc(m) labeled C2A domain of synaptotagmin I ((99)Tc(m)-Syt I-C2A) is used for noninvasive detection of vulnerable atherosclerotic plaque.</p><p><b>METHODS</b>Recombinant C2A domain of synaptotagmin I, overexpressed in E. Coli, was thiolated with 2-iminothiolane (2-IT) and labeled with (99)Tc(m). Atherosclerotic plaques were produced in 5 rabbits by deendothelialization of the abdominal aorta and the rabbits were fed with cholesterol diet for 3 months. Three rabbits not manipulated served as normal controls. All animals were injected with (99)Tc(m)-Syt I-C2A and underwent in vivo imaging thereafter. Aortas were then explanted for ex vivo imaging and histological characterization.</p><p><b>RESULTS</b>In deendothelialized animals, intense radio-uptake in abdominal aorta, showed by gamma camera at 2 h after injection, was visualized and T/B was 3.25 +/- 0.51 by ROI measurement, quantitative uptake ratio of abdominal aortas with atherosclerotic lesions to thoracic aortas was 8.39 +/- 1.74 in ex vivo imaging. The mean uptake in specimens of abdominal aortas with lesions was 12.6-fold higher than in control abdominal aortas, and 10.2-fold higher than in thoracic aortas of deendothelialized animals by gamma-counter.</p><p><b>CONCLUSION</b>(99)Tc(m)-Syt I-C2A has a high affinity for vulnerable atherosclerotic plaque and is a suitable a gent for the noninvasive detection of vulnerable atherosclerotic plaque.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Fragmentos Fab de Inmunoglobulinas
/
Diagnóstico por Imagen
/
Cintigrafía
/
Tecnecio
/
Modelos Animales de Enfermedad
/
Alergia e Inmunología
/
Aterosclerosis
/
Sinaptotagmina I
/
Marcaje Isotópico
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Cardiology
Año:
2007
Tipo del documento:
Artículo
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