Effects of a novel familial dilated cardiomyopathy associated LMNA gene mutation E82K on cell cycle of HEK293 cells / 中华心血管病杂志
Chinese Journal of Cardiology
;
(12): 21-23, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-304976
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of a novel LMNA gene mutation E82K found in a Chinese family with dilated cardiomyopathy on cell cycle of HEK293 cells.</p><p><b>METHODS</b>(1) Human wild type full-length LMNA gene cDNA was subcloned into eukaryotic expression vector pTracer-CMV and point mutation was introduced into the cDNA. LMNA gene wild type and mutant E82K LMNA gene were transfected into HEK293 cells respectively and stable cell lines resistant to antibiotic were obtained 4 weeks later. (2) Cell cycle changes were analyzed by flow cytometry in HEK293 cells transfected with wild type and mutant E82K LMNA gene and empty vector in the presence of 0.8 mmol/L H(2)O(2).</p><p><b>RESULTS</b>Cell circle was arrested at G0/G1 phase in the cells transfected with mutated E82K LMNA gene and at G2/M phase in other cell groups in the presence of H(2)O(2).</p><p><b>CONCLUSION</b>Cell circle was arrested at G0/G1 phase in the cells transfected with E82K LMNA gene in the presence of H(2)O(2) in HEK293 cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Transfección
/
Cardiomiopatía Dilatada
/
Ciclo Celular
/
Línea Celular
/
Lamina Tipo A
/
Citometría de Flujo
/
Genética
/
Mutación
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Cardiology
Año:
2007
Tipo del documento:
Artículo
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