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Regulative role of TNFalpha on STEAP4 gene in matured human adipocytes / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics ; (12): 1008-1011, 2009.
Artículo en Chino | WPRIM | ID: wpr-305133
ABSTRACT
<p><b>OBJECTIVE</b>Human STEAP4, a novel obesity-related gene, is associated with insulin sensitivity regulation in human adipocytes. This study aimed to explore the regulative role of TNFalpha on STEAP4 gene in matured human adipocytes.</p><p><b>METHODS</b>Human preadipocytes were cultured and differentiated into matured adipocytes in vitro. Fully differentiated adipocytes (Day 17) were treated with different concentrations of TNFalpha (0, 5, 10, 25 and 50 ng/mL) for 24 hrs. Total RNA and protein were extracted from the adipocytes. Levels of STEAP4 mRNA and protein expression were determined by real-time quantitative RT-PCR and Western blot respectively.</p><p><b>RESULTS</b>Different concentrations (5, 10, 25 and 50 ng/mL) of TNFalpha treatment for 24 hrs resulted in a significant increase in the STEAP4 mRNA expression of human matured adipocytes.The maximal effect was seen in the 50 ng/mL of TNFalpha treatment group. In parallel, STEAP4 protein synthesis in matured adipocytes increased in response to TNFalpha treatment of different concentrations (5, 10, 25 and 50 ng/mL) for 24 hrs. The maximal up-regulated effect was seen in the 25 ng/mL of TNFalpha treatment group.</p><p><b>CONCLUSIONS</b>TNFalpha can up-regulate STEAP4 mRNA expression in human matured adipocytes.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Oxidorreductasas / Farmacología / Proteínas Recombinantes / Células Cultivadas / Regulación de la Expresión Génica / Factor de Necrosis Tumoral alfa / Adipocitos / Relación Dosis-Respuesta a Droga / Genética / Proteínas de la Membrana Límite: Humanos Idioma: Chino Revista: Chinese Journal of Contemporary Pediatrics Año: 2009 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Oxidorreductasas / Farmacología / Proteínas Recombinantes / Células Cultivadas / Regulación de la Expresión Génica / Factor de Necrosis Tumoral alfa / Adipocitos / Relación Dosis-Respuesta a Droga / Genética / Proteínas de la Membrana Límite: Humanos Idioma: Chino Revista: Chinese Journal of Contemporary Pediatrics Año: 2009 Tipo del documento: Artículo