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Red/ET recombination and its biomedical applications / 生物工程学报
Chinese Journal of Biotechnology ; (12): 502-506, 2005.
Article en Zh | WPRIM | ID: wpr-305241
Biblioteca responsable: WPRO
ABSTRACT
Red/ET recombination, a powerful homologous recombination system based on the Red operon of lambda phage or RecE/ RecT from Rac phage, provides an innovative approach for DNA engineering. Deletion, insertion and mutation can be quickly and precisely performed on the target gene mediated by Red/ET recombination with PCR derived DNA fragments or oligonucleotides. This technical platform has extensive applications in biomedical field including bacterial artificial chromosome modification, gene knock-out construction and genetic modification of E. coli strains as well as some other kinds of microorganisms. Recently, Red/ET recombination was improved in several aspects so that it becomes more powerful and maneuverable. The characteristic and development of Red/ET recombination and its biomedical applications were described in this review.
Asunto(s)
Texto completo: 1 Índice: WPRIM Asunto principal: Rec A Recombinasas / Recombinación Genética / ADN Recombinante / Ingeniería Genética / Bacteriófago lambda / Proteínas de Escherichia coli / Proteínas de Unión al ADN / Escherichia coli / Exodesoxirribonucleasas / Genética Idioma: Zh Revista: Chinese Journal of Biotechnology Año: 2005 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Rec A Recombinasas / Recombinación Genética / ADN Recombinante / Ingeniería Genética / Bacteriófago lambda / Proteínas de Escherichia coli / Proteínas de Unión al ADN / Escherichia coli / Exodesoxirribonucleasas / Genética Idioma: Zh Revista: Chinese Journal of Biotechnology Año: 2005 Tipo del documento: Article