Red/ET recombination and its biomedical applications / 生物工程学报
Chinese Journal of Biotechnology
; (12): 502-506, 2005.
Article
en Zh
| WPRIM
| ID: wpr-305241
Biblioteca responsable:
WPRO
ABSTRACT
Red/ET recombination, a powerful homologous recombination system based on the Red operon of lambda phage or RecE/ RecT from Rac phage, provides an innovative approach for DNA engineering. Deletion, insertion and mutation can be quickly and precisely performed on the target gene mediated by Red/ET recombination with PCR derived DNA fragments or oligonucleotides. This technical platform has extensive applications in biomedical field including bacterial artificial chromosome modification, gene knock-out construction and genetic modification of E. coli strains as well as some other kinds of microorganisms. Recently, Red/ET recombination was improved in several aspects so that it becomes more powerful and maneuverable. The characteristic and development of Red/ET recombination and its biomedical applications were described in this review.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Rec A Recombinasas
/
Recombinación Genética
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ADN Recombinante
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Ingeniería Genética
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Bacteriófago lambda
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Proteínas de Escherichia coli
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Proteínas de Unión al ADN
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Escherichia coli
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Exodesoxirribonucleasas
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Genética
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2005
Tipo del documento:
Article