Progress in single-domain antibody derived from heavy-chain antibody / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 497-501, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-305242
ABSTRACT
Functional heavy-chain antibodies (HCAbs) lacking light chains occur naturally in camels. The variable domain of heavy chain of heavy-chain antibody is referred to VHH. The VHH gene family is homologous to human VH subgroup III. The single-domain VHH antibodies are constructed by cloning the variable domains of HCAbs. Compared to human VHs, VHH germ-line sequences contain some hallmark substitutions in framework region 2, including V37F(Y), G44 E, L45 R, W47G. The substitutions at positions 44, 45, 47 are often used to camelise the human VHs. Being a small binders, VHH antibodies are well expressed, extremely stable and very soluble. Camelised human VHs are proved to exhibit the same qualities as those of VHH antibodies. The single-domain VHH antibodies will be useful in the drug development and basic research.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sitios de Unión de Anticuerpos
/
Proteínas Recombinantes
/
Región Variable de Inmunoglobulina
/
Camelus
/
Genes de Inmunoglobulinas
/
Ingeniería de Proteínas
/
Cadenas Pesadas de Inmunoglobulina
/
Alergia e Inmunología
/
Genética
/
Metabolismo
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2005
Tipo del documento:
Artículo
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