Development of methods for detection of H5N1 from human clinical specimens / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 24-26, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-305534
ABSTRACT
<p><b>BACKGROUND</b>To establish a method for H5N1 RNA detection and laboratory diagnosis of suspected human avian influenza (H5N1) virus infected cases.</p><p><b>METHODS</b>The typing and sub-typing primers were designed according to M and H5 and N1 gene respectively, and the RT-PCR and real-time PCR were developed using these primers.</p><p><b>RESULTS</b>The RT-PCR and real-time PCR could be used for H5N1 detection specifically, and there was no cross reaction with other influenza subtypes such as H1 and H3. The sensitivity for RT-PCR and real-time PCR was 1 TCID50 and 0.01 TCID50 respectively. Thirteen laboratory confirmed human H5N1 cases were detected from 42 suspected cases by using these methods.</p><p><b>CONCLUSION</b>The established RT-PCR and real-time PCR methods can be used for laboratory detection of suspected human H5N1 cases.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Virales
/
Virología
/
Reproducibilidad de los Resultados
/
Sensibilidad y Especificidad
/
Cartilla de ADN
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Diagnóstico
/
Gripe Humana
/
Subtipo H5N1 del Virus de la Influenza A
/
Genética
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2006
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS