Expression and characterization of transcutaneous immunization adjuvant LTB and LTK63 / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>BACKGROUND</b>To study a new kind of adjuvant: transcutaneous immunization adjuvant.</p><p><b>METHODS</b>The full length gene of Heat-labile enterotoxin (LT) was amplified from E. coli H10407. The B subunit protein LTB and the nontoxic A subunit protein LTKA were expressed by genetic engineering manipulation. After purification, they were identified with SDS-PAGE, GM1-ELISA and so on.</p><p><b>RESULTS</b>The LTB protein still persisted its biologic activity that conjugated specifically with GM1 ganglioside, and the LTK63 protein lost its toxin activity.</p><p><b>CONCLUSION</b>The results showed that LTB and LTK63 may be used as promising transcutaneous immunization adjuvant.</p>