Effect of endothelin-1 and its antagonists on the expression of endothelin receptors mRNA in HSC-T6 cells / 中华外科杂志
Chinese Journal of Surgery
;
(12): 1395-1397, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-306101
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of endothelin-1 (ET-1) and its antagonists on the expression of endothelin and its receptors mRNA in HSC-T6 cells.</p><p><b>METHODS</b>Cultured HSC-T6 cells were randomly divided into 7 groups Sham control group, ET-1 group (10 nmol/L ET-1), BQ-123 group [1 micromol/L BQ-123, a selective endothelin receptor A (ETRA) antagonist], BQ-788 group [1 micromol/L BQ-788, a selective endothelin receptor B (ETRB) antagonist], ET-1 + BQ123 group (10 nmol/L ET-1 + 1 micromol/L BQ-123), ET-1 + BQ-788 group (10 nmol/L ET-1 + 1 micromol/L BQ-788) and ET-1 + BQ-788 group (10 nmol/L ET-1 + 1 micromol/L BQ-123 + 1 micromol/L BQ-788). The expression of endothelin receptor mRNA of HSC-T6 cells was determined by reverse-transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>The expression of ETRA mRNA in ET-1 + BQ123 + BQ788 and ET-1 + BQ788 group was significantly lower than ET-1 group (0.329 +/- 0.044 and 0.292 +/- 0.023 vs. 0.440 +/- 0.030 P < 0.05). Compared with ET-1 group, the expression of ETRB mRNA in ET-1 + BQ788 group was down regulated obviously (0.499 +/- 0.136 vs. 0.153 +/- 0.071, P < 0.05). There was no significant difference in ET-1 + BQ123 group and ET-1 + BQ123 + BQ788 group when compared with ET-1 group (0.499 +/- 0.136 vs. 0.496 +/- 0.103 and 0.299 +/- 0.129, P > 0.05).</p><p><b>CONCLUSIONS</b>ET-1 has no obvious effect on the expression of ETRA mRNA in HSC-T6. ET-1 may up-regulate the expression of ETRB mRNA. Act on ETRA receptor, ET-1 can inhibit the expression of ETRB mRNA.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oligopéptidos
/
Péptidos Cíclicos
/
Farmacología
/
Piperidinas
/
ARN Mensajero
/
Células Cultivadas
/
Regulación de la Expresión Génica
/
Ratas Sprague-Dawley
/
Endotelina-1
/
Hepatocitos
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Surgery
Año:
2005
Tipo del documento:
Artículo
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