Screening of differential proteins binding to Nox1 promoter in A549 cell model of inflammation and oxidative stress / 南方医科大学学报
Journal of Southern Medical University
;
(12): 703-707, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-306484
ABSTRACT
<p><b>OBJECTIVE</b>To screen the regulatory proteins involved in Nox1 promoter activation in a cell model of inflammation and oxidative stress.</p><p><b>METHODS</b>A cell model of inflammation and oxidative stress was established by stimulating A549 cells with tumor necrosis factor-α (TNF-α). The differential proteins binding to Nox1 promoter were screened by DNA pull-down and the binding proteins were separated by 2D electrophoresis and selected according to the their differential expression levels (with over 1.5-fold changes relative to the control level). The screened proteins were finally identified by MALDI-TOF/TOF-MS.</p><p><b>RESULTS</b>Seven differentially expressed protein spots (all upregulated in the cell model) were obtained, among which GLE1, DDX19A, KRT1 and KRT10 were identified by mass spectrometry.</p><p><b>CONCLUSION</b>GLE1, DDX19A, KRT1 and KRT10 participate in the activation of Nox1 promoter in TNF-α-induced A549 cells, and this result provides new insights into the biological roles of the regulatory proteins of Nox1 promoter in inflammation and oxidative stress.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Espectrometría de Masas
/
Electroforesis en Gel Bidimensional
/
Regiones Promotoras Genéticas
/
Factor de Necrosis Tumoral alfa
/
Estrés Oxidativo
/
NADPH Oxidasas
/
Proteínas de Transporte Nucleocitoplasmático
/
Línea Celular Tumoral
/
Queratina-10
/
Queratina-1
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
/
Estudio de tamizaje
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2013
Tipo del documento:
Artículo
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