Changes in gene expression profiles in multiple myeloma cells after knocking-down SNF5, a core subunit of SWI/SNF complex / 南方医科大学学报
Journal of Southern Medical University
;
(12): 667-671, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-306490
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes in the gene expression profiles in a multiple myeloma cell line after knocking-down the expression of SNF5, a core component of the SWI/SNF chromatin remodeling complex.</p><p><b>METHODS</b>The total RNA were extracted from tetracycline-inducible SNF5 knockdown (KD) cell line derived from KM3 cells for gene expression profiling by affymetrix microarray and bioinformatic analysis.</p><p><b>RESULTS</b>Knockdown of SNF5 inhibited KM3 cell proliferation. A total of 545 genes were found to be differentially expressed in the cells with SNF5 knockdown, among which 214 were up-regulated and 331 were down-regulated.</p><p><b>CONCLUSIONS</b>SNF5 is essential for the growth of multiple myeloma cells and can regulate the expression of the genes associated with cell growth and apoptosis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Factores de Transcripción
/
Proteínas Cromosómicas no Histona
/
Regulación Neoplásica de la Expresión Génica
/
Apoptosis
/
ARN Interferente Pequeño
/
Línea Celular Tumoral
/
Proliferación Celular
/
Proteínas de Unión al ADN
/
Técnicas de Silenciamiento del Gen
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2013
Tipo del documento:
Artículo
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