The influence of the recombinant lentiviral vectors encoding Hyper-IL-6 on the proliferation of human hepatic cell / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 615-619, 2009.
Article
en Zh
| WPRIM
| ID: wpr-306723
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Hyper-IL-6 on the proliferation of L-02 cells.</p><p><b>METHODS</b>The recombinant lentiviral vectors encoding Hyper-IL-6 (FIV-Hyper-IL-6), IL-6 (FIV-IL-6) and the lentiviral (FIV) were prepared using the FIV-based lentiviral vectors and the packaging system. The titer of FIV-Hyper-IL-6, FIV-IL-6 and FIV was tested with puromycin. L-02 cells were infected with FIV-Hyper-IL-6, FIV-IL-6, FIV, or mock-infected. The growth rate of L02 cells was analyzed with MTT at different time points after infection. The changes of Haptoglobin in L-02 cells were analyzed with RT-PCR.</p><p><b>RESULTS</b>FIV-Hyper-IL-6, FIV-IL-6 and FIV were successfully constructed, and the titer was 107 pfu/ml. 48 hours after infection, the absorbance of the cells infected with FIV-Hyper-IL-6 was 0.6267+/-0.0256, and the absorbances of FIV-IL-6 infected cells, FIV infected cells and mock-infected cells were 0.5563+/-0.0112, 0.5040+/-0.0078 and 0.4790+/-0.0201, respectively. There were significant differences between the FIV-Hyper-IL-6 group and the other groups (F = 41.09, P less than 0.01). The ratio of the absorbance between haptoglobin mRNA and beta-actin was 0.7030+/-0.0106, 0.3355+/-0.0093, 0.1145+/-0.0076 and 0.1143+/-0.0153, respectively, in FIV-Hyper-IL-6 infected cells, FIV-IL-6 infected cells, FIV infected cells and mock-infected cells. There were significant differences between the FIV-Hyper-IL-6 group and the others (q = 57.5007, P less than 0.01).</p><p><b>CONCLUSION</b>Compared with IL-6, Hyper-IL-6 is more potent to stimulate proliferation and induce the expression of Haptoglobin in L-02 cells.</p>
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Índice:
WPRIM
Asunto principal:
Factores de Tiempo
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Proteínas Recombinantes de Fusión
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Haptoglobinas
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ARN Mensajero
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Transfección
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Interleucina-6
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Lentivirus
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Hepatocitos
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Biología Celular
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Año:
2009
Tipo del documento:
Article