Quantitative analysis of adenosine and cordycepin in Cordyceps sinensis and its substitutes with LC-MS-MS / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2018-2021, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-307540
ABSTRACT
<p><b>OBJECTIVE</b>To develop a LC-MS-MS method for determination of adenosine and cordycepin in Cordyceps sinensis and it's substitutes.</p><p><b>METHOD</b>The sawple was extracted with. 90% methanol. Multi-reactions monitoring (MRM) technique was adopted.</p><p><b>RESULT</b>The regression equations and coefficients were Y = 89.04X + 506.85 (r = 0.999 7) for adenosine, Y = 99.66X + 1 251.34 (r = 0.998 8) for cordycepin respectively. The linear range was 5.0-1 000.0 microg x L(-1) for adenosine and cordycepin. The limits of detection (LOD) were 0. 44 microg x L(-1) for adenosine and 0.31 microg x L(-1) for cordycepin, respectively. The average recoveries of adenosine and cordycepin were 98.1% and 97.9%, respectively.</p><p><b>CONCLUSION</b>The method was highly sensitive, selective and fast, which can be used for the determination of adenosine and cordycepin in C. sinensis and it's substitutes. This method can also be applied for the quality control of the medicinal materials.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Control de Calidad
/
Estándares de Referencia
/
Valores de Referencia
/
Adenosina
/
Química
/
Desoxiadenosinas
/
Reproducibilidad de los Resultados
/
Cromatografía Líquida de Alta Presión
/
Clasificación
/
Espectrometría de Masa por Ionización de Electrospray
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2007
Tipo del documento:
Artículo
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