Nitidine chloride-induced apoptosis of human osteosarcoma cells and its mechanism / 南方医科大学学报
Journal of Southern Medical University
;
(12): 361-364, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-307931
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis-inducing effect of nitidine chloride in human osteosarcoma MG-63 cells and explore its mechanism.</p><p><b>METHODS</b>The effect of nitidine chloride on the proliferation of MG-63 cells was detected by colorimetric MTT assay, and the morphological changes of cells treated with nitidine chloride were observed using fluorescence and electron microscope. Flow cytometry was performed to analyze the apoptotic rate of the cells, and the protein expression levels of caspase-3, caspase-9, Bcl-2 and Bax were detected by Western blotting.</p><p><b>RESULTS</b>Nitidine chloride inhibited the proliferation of MG-63 cells in a dose- and time-dependent manner. Fluorescence and electron microscopy revealed distinct apoptotic changes of the cells after nitidine chloride exposure. Flow cytometry indicated that nitidine chloride induced the apoptosis of MG-63 cells in a dose-dependent manner. Exposure to nitidine chloride, as shown by Western blotting, resulted in increased expressions of cleaved caspase-3, cleaved caspase-9 and Bax and decreased expressions of pro-caspase-3, pro-caspase-9 and Bcl-2.</p><p><b>CONCLUSION</b>Nitidine chloride can inhibit the proliferation of osteosarcoma cell line MG-63 by inducing cell apoptosis, the mechanism of which might be related with the activation of the caspase-dependent pathway.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Neoplasias Óseas
/
Osteosarcoma
/
Apoptosis
/
Línea Celular Tumoral
/
Relación Dosis-Respuesta a Droga
/
Benzofenantridinas
/
Antineoplásicos Fitogénicos
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2011
Tipo del documento:
Artículo
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