ClC-3 siRNA inhibits regulatory volume decrease in nasopharyngeal carcinoma cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 216-220, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-307966
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of ClC-3 chloride channels in regulatory volume decrease (RVD) of nasopharyngeal carcinoma (NPC) CNE-2Z cells.?</p><p><b>METHODS</b>ClC-3 siRNA was transfected into CNE-2Z cells in the presence of the transfection reagent HiPerFect Reagent(TM). The transfection efficiency of ClC-3 siRNA was detected by flow cytometry. The expression of ClC-3 protein was detected by Western blotting, and the changes of cell volume in 160 mOsmol/L hypotonic solution were determined by image analysis.</p><p><b>RESULTS</b>The transfection efficiency of ClC-3 siRNA was (63.8∓3.8)% (n=3, P<0.01), and compared with the control group, ClC-3 siRNA transfection resulted in a reduction of ClC-3 expression by (60.9∓4.0)% (n=3, P<0.01). The hypotonic challege (160 mOsmol/L) caused cell swelling and induced RVD. In the control group, hypotonic solution bath for 35 min resulted in a RVD of (42.6∓2.8)% (n=20), which was significantly decreased to (10.5∓4.8)% (n=16) in ClC-3 siRNA-transfected cells, demonstrating a reduction of RVD capacity by 75.4% (P<0.01).?</p><p><b>CONCLUSION</b>The capacity of RVD is significantly reduced in CNE-2Z cells by ClC-3 chloride channel protein knock-down via ClC-3 siRNA transfection, indicating an important role of ClC-3 chloride channels in the RVD of CNE-2Z cells.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Transfección
/
Neoplasias Nasofaríngeas
/
Canales de Cloruro
/
ARN Interferente Pequeño
/
Interferencia de ARN
/
Línea Celular Tumoral
/
Tamaño de la Célula
/
Genética
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2011
Tipo del documento:
Artículo
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