Cholecystokinin octapeptide inhibits the in vitro expression of CD14 in rat pulmonary interstitial macrophages induced by lipopolysaccharide / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 276-279, 2002.
Article
en En
| WPRIM
| ID: wpr-308102
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of cholecystokinin octapeptide (CCK-8) on lipopolysaccharide (LPS)-stimulated pulmonary interstitial macrophages (PIM) in vitro.</p><p><b>METHODS</b>PIM were isolated and cultured in the presence or absence of LPS, CCK-8, proglumide (the antagonist of CCK receptors) and vehicle. The expression of membrane CD14 (mCD14) protein was assayed by flow cytometry and soluble CD14 (sCD14) in the supernatant was analyzed semi-quantitatively by Western blot. TNF-alpha in the supernatant was detected with ELISA.</p><p><b>RESULTS</b>CCK-8, at concentrations of 10(-7) mol/L and 10(-6) mol/L, significantly inhibited the expression of mCD14. Release of sCD14 and TNF-alpha in the supernatant was up-regulated by LPS (1 microg/ml) but reduced by CCK-8. The effect of CCK-8 was inhibited by proglumide.</p><p><b>CONCLUSION</b>CCK-8 negatively modulated several functions of LPS-stimulated PIM through CCK receptors. This may be one of the mechanisms for CCK-8 to alleviate inflammation in lung tissue during endotoxemia.</p>
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WPRIM
Asunto principal:
Farmacología
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Sincalida
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Células Cultivadas
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Química
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Lipopolisacáridos
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Factor de Necrosis Tumoral alfa
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Macrófagos Alveolares
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Ratas Sprague-Dawley
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Medios de Cultivo Condicionados
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Organismos Libres de Patógenos Específicos
Límite:
Animals
Idioma:
En
Revista:
Chinese Medical Journal
Año:
2002
Tipo del documento:
Article