Content determination of two isomers containd in Garcinia hanburyi by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method for determing the content of two isomers containd in Garcinia hanburyi by HPLC.</p><p><b>METHOD</b>Chromatographic column of SunFire (Waters) C8 (2.1 mm x 150 mm, 3.5 microm) was adopted, with acetonitrile-methanol-0.3% trifluoroacetic acid (36 3727) as the mobile phase. The detection wavelength was 360 nm,the flow rate was 0.3 mL x min(-1), and the column temperature was 28 degrees C.</p><p><b>RESULT</b>The linear regression equation of r-gambogic acid was Y = 2.87 x 10(6) X - 2.24 x 10(5), r = 0.999 9. The linear regression equation of S-gambogic acid was Y = 3.31 x 10(6) X - 1.44 x 10(5), r = 0.999 9. The average recoveries were 100.0% and 100.9%, with RSD being 2.1% and 2.5% (n = 6), respectivley. The average contents of two gambogic acid in G. hanburyi were 30.06% and 21.45%, respectively.</p><p><b>CONCLUSION</b>The method was so convenient and stable that it can be used for identification and content determination of two isomers containd in G. hanburyi.</p>