Study on norcantharidin-induced apoptosis in SMMC-7721 cells through mitochondrial pathways / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 448-452, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-308738
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanism of norcantharidin (NCTD)-induced SMMC-7721 hepatoma cell apoptosis.</p><p><b>METHODS</b>SMMC-7721 cell growth inhibition was measured by the MTT method. Apoptosis was detected by Annexin V/propidium iodide staining. The mitochondrial membrane potential was measured by flow cytometry. Western blot analysis was used to evaluate the level of cytochrome c, caspase-3, AIF, Bcl-2 and Bax expression.</p><p><b>RESULTS</b>NCTD inhibited SMMC-7721 cell growth in a time- and dose-dependent manner. The cells treated with NCTD showed the loss of mitochondrial membrane potential. The activities of caspase-3, cytochrome c, AIF, and Bax were up-regulated after NCTD treatment at different doses. The expression of Bcl-2 was decreased after treatment with NCTD.</p><p><b>CONCLUSIONS</b>NCTD could induce SMMC-7721 cell apoptosis. The activation of the mitochondrial pathway was involved in the process of NCTD-induced SMMC-7721 cell apoptosis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Western Blotting
/
Apoptosis
/
Compuestos Bicíclicos Heterocíclicos con Puentes
/
Línea Celular Tumoral
/
Citocromos c
/
Factor Inductor de la Apoptosis
/
Proteína X Asociada a bcl-2
/
Caspasa 3
Límite:
Humanos
Idioma:
Inglés
Revista:
Chinese journal of integrative medicine
Año:
2010
Tipo del documento:
Artículo
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