Species-specific SCAR markers for authentication of Sinocalycanthus chinensis / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 868-872, 2006.
Artículo
en Inglés
| WPRIM
| ID: wpr-309061
ABSTRACT
Sinocalycanthus chinensis, an endangered species endemic to China, is cultivated as an ornamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthus floridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR (inter-simple sequence repeats) were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR (sequence characterized amplified regions) markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs of S. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid (results can be obtained in less than 3 h), but is also easy to perform. Hence it is a feasible method for identifying S. chinensis in seedling market.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Especificidad de la Especie
/
Marcadores Genéticos
/
Hojas de la Planta
/
ADN de Plantas
/
Técnica del ADN Polimorfo Amplificado Aleatorio
/
Calycanthaceae
/
Genética
Idioma:
Inglés
Revista:
Journal of Zhejiang University. Science. B
Año:
2006
Tipo del documento:
Artículo
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