Growth factors promote the survival and proliferation of human spermatogonium stem cells in vitro / 中华男科学杂志
National Journal of Andrology
;
(12): 876-878, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-309758
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of growth factors on the survival and proliferation of human spermatogonial stem cells (SSCs) in vitro.</p><p><b>METHODS</b>SSCs were treated with the growth factors SCF, LIF and bFGF added to the culture, each at the concentrations of 0, 5, 10 and 20 microg/L and repeated three times. The survival time and proliferation rate of the cells were determined every 8-12 hours and their morphological features observed with the light microscope and electron microscope.</p><p><b>RESULTS</b>The survival time and proliferation rate of the SSCs were significantly increased in the treated groups as compared with the control (P < 0.05).</p><p><b>CONCLUSION</b>The growth factors SCF, LIF and bFGF can promote the survival and proliferation of SSCs in vitro.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Espermatogonias
/
Células Madre
/
Supervivencia Celular
/
Células Cultivadas
/
Factor 2 de Crecimiento de Fibroblastos
/
Factor de Células Madre
/
Biología Celular
/
Péptidos y Proteínas de Señalización Intercelular
/
Proliferación Celular
Límite:
Humanos
/
Masculino
Idioma:
Chino
Revista:
National Journal of Andrology
Año:
2008
Tipo del documento:
Artículo
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