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Growth factors promote the survival and proliferation of human spermatogonium stem cells in vitro / 中华男科学杂志
National Journal of Andrology ; (12): 876-878, 2008.
Artículo en Chino | WPRIM | ID: wpr-309758
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of growth factors on the survival and proliferation of human spermatogonial stem cells (SSCs) in vitro.</p><p><b>METHODS</b>SSCs were treated with the growth factors SCF, LIF and bFGF added to the culture, each at the concentrations of 0, 5, 10 and 20 microg/L and repeated three times. The survival time and proliferation rate of the cells were determined every 8-12 hours and their morphological features observed with the light microscope and electron microscope.</p><p><b>RESULTS</b>The survival time and proliferation rate of the SSCs were significantly increased in the treated groups as compared with the control (P < 0.05).</p><p><b>CONCLUSION</b>The growth factors SCF, LIF and bFGF can promote the survival and proliferation of SSCs in vitro.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Espermatogonias / Células Madre / Supervivencia Celular / Células Cultivadas / Factor 2 de Crecimiento de Fibroblastos / Factor de Células Madre / Biología Celular / Péptidos y Proteínas de Señalización Intercelular / Proliferación Celular Límite: Humanos / Masculino Idioma: Chino Revista: National Journal of Andrology Año: 2008 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Espermatogonias / Células Madre / Supervivencia Celular / Células Cultivadas / Factor 2 de Crecimiento de Fibroblastos / Factor de Células Madre / Biología Celular / Péptidos y Proteínas de Señalización Intercelular / Proliferación Celular Límite: Humanos / Masculino Idioma: Chino Revista: National Journal of Andrology Año: 2008 Tipo del documento: Artículo