Effects of 8-Methoxypsoralen on intracellular Ca(2+)i and cytoskeleton actin organization in human melanocytes in vitro / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of 8-methoxypsoralen on human melanocytes [Ca(2+)]i and cytoskeleton actin organization in vitro.</p><p><b>METHODS</b>Human melanocytes were obtained from normal foreskins. Laser confocal microscope was employed to measure [Ca(2+)]i and rhodamine-conjugated phalloidin was used to visualize the cytoskeleton actin.</p><p><b>RESULTS</b>8-methoxypsoralen increased [Ca(2+)]i and induced organization of actin stress fiber cytoskeleton.</p><p><b>CONCLUSION</b>8-methoxypsoralen might influence the migration of melanocytes by increasing the intracellular free Ca(2+) concentration and cytoskeleton actin reorganization.</p>