Effect of environment temperature on the recovery of microdialysis probe for monoamine neurotransmitter / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of environment temperature on the recovery of microdialysis probe for neurotransmitters.</p><p><b>METHODS</b>Neurotransmitters NE, DA, and 5-HT were dialyzed with 4 mm membrane length microdialysis probes in different environmental temperature. There were three conditions lower temperature condition, the temperature of standard solution and that of the perfusate were both 24 degree; higher temperature condition,both were 37.5 degree; and the middle temperaturethe perfusate was 24 degree and the standard solution was 37.5 degree. The concentrations of neurotransmitters in the dialyzed solution and the standard solution were analyzed by HPLC-ECD, and recoveries were then calculated.</p><p><b>RESULT</b>In lower temperature condition,the recoveries of microdialysis probe for NE, DA, 5-HT were 18.3 %, 19.6% and 16.9%, respectively. In middle temperature condition, the recoveries were 29.6%, 30.7% and 24.3%, respectively, and in higher temperature condition, those were 49.2%, 47.5% and 37.2%, respectively. With the analysis of variance, the recoveries for NE, DA, 5-HT increased with temperature significantly (P<0.01).</p><p><b>CONCLUSION</b>Both the perfusate and the standard solution affects the environmental temperature of microdialysis probe, which in turns affects the recovery of microdialysis probe for neurotransmitters. So in order to calculate the recovery more accurately, the standard solution/the perfusate should be kept in body temperature.</p>