Cortical neuron apoptosis induced by beta-amyloid peptide and protective effect of panoxadiol in mice / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 451-455, 2008.
Article
en Zh
| WPRIM
| ID: wpr-310432
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the apoptosis of cortical neurons induced by beta-amyloid peptide (Abeta(1-40)) and the protective effect of panoxadiol.</p><p><b>METHODS</b>The Abeta(1-40) induced damage of primarily cultured mouse cortical neurons was examined with morphological observation, MTT assay, DNA agarose gel electrophoresis and Western-blot.</p><p><b>RESULT</b>After 48 h treated with 12 mumol/L Abeta(1-40), the cortical neurons showed apoptotic characteristics: including decreased OD570 value in MTT assay, DNA cleavage fragment in electrophoresis and increased apoptotic cells. Western-blot showed that the expression of bcl-2 reduced significantly (P<0.05). Cell apoptosis was significantly attenuated in 40 mg/L panoxadiol treated group.</p><p><b>CONCLUSION</b>Panoxadiol can protect cultured cortical neurons from apoptosis induced by Abeta(1-40) in mice.</p>
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Índice:
WPRIM
Asunto principal:
Fragmentos de Péptidos
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Farmacología
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Medicamentos Herbarios Chinos
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Células Cultivadas
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Corteza Cerebral
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Péptidos beta-Amiloides
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Apoptosis
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Fármacos Neuroprotectores
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Proteínas Proto-Oncogénicas c-bcl-2
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Biología Celular
Límite:
Animals
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Pregnancy
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2008
Tipo del documento:
Article