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Expression of Potassium Channels in Uterine Smooth Muscle Cells from Patients with Adenomyosis / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 200-205, 2016.
Artículo en Inglés | WPRIM | ID: wpr-310683
ABSTRACT
<p><b>BACKGROUND</b>Adenomyosis (AM) has impaired contraction. This study aimed to explore the expression of potassium channels related to contraction in myometrial smooth muscle cells (MSMCs) of AM.</p><p><b>METHODS</b>Uterine tissue samples from 22 patients (cases) with histologically confirmed AM and 12 (controls) with cervical intraepithelial neoplasia were collected for both immunohistochemistry and real-time polymerase chain reaction to detect the expression of large conductance calcium- and voltage-sensitive K + channel (BKCa)-α/β subunits, voltage-gated potassium channel (Kv) 4.2, and Kv4.3. Student's t-test was used to compare the expression.</p><p><b>RESULTS</b>The BKCa-α/β subunits, Kv4.2, and Kv4.3 were located in smooth muscle cells, glandular epithelium, and stromal cells. However, BKCa-β subunit expression in endometrial glands of the controls was weak, and Kv4.3 was almost undetectable in the controls. The expression of BKCa-α messenger RNA (mRNA) (0.62 ± 0.19-fold decrease, P < 0.05) and Kv4.3 mRNA (0.67 ± 0.20-fold decrease, P < 0.05) decreased significantly in the MSMCs of the control group compared with the AM group. However, there were no significant differences in BKCa-β subunit mRNA or Kv4.2 mRNA.</p><p><b>CONCLUSIONS</b>The BKCa-α mRNA and the Kv4.3 mRNA are expressed significantly higher in AM than those in the control group, that might cause the abnormal uterus smooth muscle contractility, change the microcirculation of uterus to accumulate the inflammatory factors, impair the endometrium further, and aggravate the pain.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Contracción Uterina / Neoplasias Uterinas / Útero / Inmunohistoquímica / Canales de Potasio con Entrada de Voltaje / Miocitos del Músculo Liso / Canales de Potasio de Gran Conductancia Activados por el Calcio / Canales de Potasio Shal / Reacción en Cadena en Tiempo Real de la Polimerasa Límite: Adulto / Femenino / Humanos / Masculino Idioma: Inglés Revista: Chinese Medical Journal Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fisiología / Contracción Uterina / Neoplasias Uterinas / Útero / Inmunohistoquímica / Canales de Potasio con Entrada de Voltaje / Miocitos del Músculo Liso / Canales de Potasio de Gran Conductancia Activados por el Calcio / Canales de Potasio Shal / Reacción en Cadena en Tiempo Real de la Polimerasa Límite: Adulto / Femenino / Humanos / Masculino Idioma: Inglés Revista: Chinese Medical Journal Año: 2016 Tipo del documento: Artículo