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Effects of delta-opioid receptor stimulation on survival of cultured myocardial cells upon to serum deprivation / 中国应用生理学杂志
Chinese Journal of Applied Physiology ; (6): 274-278, 2008.
Artículo en Chino | WPRIM | ID: wpr-310747
ABSTRACT
<p><b>AIM</b>To study upon to serum deprivation if delta-opioid receptor activation has direct effect on cultured impaired cardiomyocytes survival.</p><p><b>METHODS</b>Myocardial cells of neonatal rats were cultured in vitro. The cell viability was determined with crystal violet staining uptake. The percentage of S + G2 + M in cell cycle was determined by flow cytometry. Apoptosis rates were determined by flow cytometry (FCM). The expression of Caspase-3 were investigated by Western blotting.</p><p><b>RESULTS</b>Myocardial cells of neonatal rats were cultured of serum-free in vitro, apoptotic index was significantly increased, the expression of Caspase-3 was significantly increased, free-serum induced apoptosis in cardiac myocytes after 48 h. At concentrations of 10 nmol x L(-1) - 10 micromol x L(-1), a delta opoid receptor agonist [D-Ala2, D-Leu5]-enkephalin DADLE promoted the myocardial cells survival, in a concentration-dependent manner. The optimal response was achieved at 0.1 micromol x L(-1), which increase survival index of cardiac myocyte, percentage of S + G2 + M in cell cycle, decrease apoptotic index of cardiac myocyte, and the expression activate caspase-3. Delta-opioid receptor antagonist naltrindole at 10 micromol x L(-1) inhibited the promoting effects of DADLE, which decrease survival index of cardiac myocyte, and percentage of S + G2 + M in cell cycle, increase apoptotic index of cardiac myocyte and the expression of Caspase-3.</p><p><b>CONCLUSION</b>The protective of delta-opioid receptor activation can promote survival in cultured impaired myocardial cells.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Supervivencia Celular / Células Cultivadas / Leucina Encefalina-2-Alanina / Medio de Cultivo Libre de Suero / Ratas Sprague-Dawley / Apoptosis / Receptores Opioides delta / Biología Celular / Miocitos Cardíacos Límite: Animales Idioma: Chino Revista: Chinese Journal of Applied Physiology Año: 2008 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Supervivencia Celular / Células Cultivadas / Leucina Encefalina-2-Alanina / Medio de Cultivo Libre de Suero / Ratas Sprague-Dawley / Apoptosis / Receptores Opioides delta / Biología Celular / Miocitos Cardíacos Límite: Animales Idioma: Chino Revista: Chinese Journal of Applied Physiology Año: 2008 Tipo del documento: Artículo