Establishment of the coculture systems of rabbit aortic endothelial cells and smooth muscle cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 509-512, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-310822
ABSTRACT
<p><b>AIM</b>In order to establish a coculture system of ECs and SMCs and by which further study can be done.</p><p><b>METHODS</b>ECs in primary culture were grown on a side of Transwell membrane, and SMCs were grown on an other side of it or the bottom of culture well, so that two kinds of coculture systems were established, and detail observation on the coculture systems was carried out by transmission and scanning electron microscope.</p><p><b>RESULTS</b>ECs in primary culture were positive of VI factor by immunocytochemistry staining. ECs and SMCs were grown well on both sides of Transwell membrane, relative to ECs monolayer of "cobblestone appearance", SMCs were multilayer of "hills and valleys appearance". ECs and SMCs on both sides of Transwell membrane could form the gap junctions by micropores.</p><p><b>CONCLUSION</b>The coculture systems of ECs and SMCs were established successfully by modeling the structural relationship of vascular wall.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Aorta
/
Endotelio Vascular
/
Comunicación Celular
/
Técnicas de Cocultivo
/
Biología Celular
/
Miocitos del Músculo Liso
/
Células Endoteliales
/
Músculo Liso Vascular
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2007
Tipo del documento:
Artículo
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