Effect of Salvia miltiorrhiza monomer IH764-3 on MMP-13 and TIMP-1 by downregulating the expression of focal adhesion kinase in hepatic stellate cell stimulated by H2O2 / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 482-486, 2007.
Article
en Zh
| WPRIM
| ID: wpr-310828
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate the effect of IH764-3 on the expression of MMP-13 and TIMP-1 by H2O2-stimulated hepatic stellate cell and the alteration of FAK during this process.</p><p><b>METHODS</b>The expression of MMP-13 and FAK mRNA was examined by RT-PCR. TIMP-1 mRNA was analyzed by in-situ hybridization. FAK and TIMP-1 were evaluated at protein level through Western blotting method.</p><p><b>RESULTS</b>Being incubated for 2 h, compared with control group, MMP-13 mRNA was upregulated by IH764-3, but TIMP-1 transcription was reduced in a dose-dependent manner, accompanied with the decrease of FAK mRNA. The expression of TIMP-1 and FAK protein in HSC also decreased after being exposed by IH764-3 for 24 h.</p><p><b>CONCLUSION</b>IH764-3 can induce the expression of MMP-13 and inhibit the expression of TIMP-1. Down-regulating the expression of FAK mRNA may be one of its mechanisms.</p>
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Índice:
WPRIM
Asunto principal:
Farmacología
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Medicamentos Herbarios Chinos
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Regulación hacia Abajo
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Células Cultivadas
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Química
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Inhibidor Tisular de Metaloproteinasa-1
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Salvia miltiorrhiza
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Proteína-Tirosina Quinasas de Adhesión Focal
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Metaloproteinasa 13 de la Matriz
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Células Estrelladas Hepáticas
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2007
Tipo del documento:
Article