Myelo-Enhancement by Astragalus Membranaceus in Male Albino Rats with Chemotherapy Myelo-Suppression. Histological and Immunohistochemical Study
International Journal of Stem Cells
;
: 12-22, 2014.
Artículo
en Inglés
| WPRIM
| ID: wpr-31120
ABSTRACT
BACKGROUND AND OBJECTIVES:
Myelo-suppression is the most common toxicity encountered in the oncology clinic today. This study was planned to investigate the possible protective and therapeutic role of the traditional Chinese Medicinal Herb; Astragalus Membranaceus (AM), on chemotherapy-induced myelosuppression. METHODS ANDRESULTS:
This study was carried out on thirty six adult male albino rats. They were divided into Group I Control Group (n=6) received a vehicle of phosphate buffered saline (PBS) solution. Group II (n=12) were injected I.P. with cyclophosphamide (CY) for 3 days (gIIa n =6) and continued for one more week to receive AM orally (gIIb n=6). Group III (n=6) received CY I.P. together with AM orally for 3 days. Group IV (n=12) received AM orally for one week (gIVa n=6) and continued for extra three days receiving CY I.P. with AM orally (gIVb n=6). Blood samples were analysed for Total Leucocytic Count and Lymphocytic Count. Counting of CD34 +ve cells in bone marrow was performed by flowcytometry. Bone marrow sections were subjected to H&E stain as well as immunohistochemical staining for anti- CD20 antibody. The mean area % of cellular bone marrow regions occupied by developing haemopoietic cells, mean area of fat cells and mean number of CD20 immunopositive B lymphocytes in the bone marrow were measured by histomorphometric studies and statistically compared. AM proved to have a myelo-protective and myelo-therapeutic capacity, evidenced at both laboratory and morphological levels.CONCLUSIONS:
The greatest myelo-potentiating effect of AM was achieved when supplied before and together with CY therapy.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plantas Medicinales
/
Médula Ósea
/
Linfocitos B
/
Adipocitos
/
Ciclofosfamida
/
Astragalus propinquus
/
Pueblo Asiatico
/
Quimioterapia
Límite:
Animales
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
International Journal of Stem Cells
Año:
2014
Tipo del documento:
Artículo
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