Role of Endoplasmic Reticulum Stress in Silica-induced Apoptosis in RAW264.7 Cells / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 591-600, 2017.
Artículo
en Inglés
| WPRIM
| ID: wpr-311374
ABSTRACT
<p><b>OBJECTIVE</b>We investigated the role of endoplasmic reticulum stress (ERS) in silica-induced apoptosis in alveolar macrophages in vitro.</p><p><b>METHODS</b>RAW264.7 cells were incubated with 200 μg/mL silica for different time periods. Cell viability was assayed by the MTT assay. Cell apoptosis was evaluated by DAPI staining, flow cytometry analysis, and Western blot analysis of caspase-3. Morphological changes in the endoplasmic reticulum were observed by transmission electron microscopy. The expression of ERS markers binding protein (BiP) and CCAAT-enhancer-binding protein homologous protein (CHOP) was examined by Western blotting and real-time PCR. As an inhibitor of ERS, 4-phenylbutyric acid (4-PBA) was used in the experiments.</p><p><b>RESULTS</b>Silica exposure induced nuclear condensation and caspase-3 expression in RAW264.7 cells. The number of apoptotic cells increased after silica exposure in a time-dependent manner. Silica treatment induced expansion of the endoplasmic reticulum. In addition, the expression of BiP and CHOP increased in silica-stimulated cells. Furthermore, 4-PBA treatment inhibited silica-induced endoplasmic reticulum expansion and the expression of BiP and CHOP. Moreover, 4-PBA treatment attenuated nuclear condensation, reduced apoptotic cells, and downregulated caspase-3 expression in silica-stimulated cells.</p><p><b>CONCLUSION</b>Silica-induced ERS is involved in the apoptosis of alveolar macrophages.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Butilaminas
/
Supervivencia Celular
/
Apoptosis
/
Dióxido de Silicio
/
Toxicidad
/
Estrés del Retículo Endoplásmico
Límite:
Animales
Idioma:
Inglés
Revista:
Biomedical and Environmental Sciences
Año:
2017
Tipo del documento:
Artículo
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