Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1262-1264, 2003.
Artículo
en Inglés
| WPRIM
| ID: wpr-311704
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases.</p><p><b>METHODS</b>Vero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells.</p><p><b>RESULTS</b>Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect.</p><p><b>CONCLUSION</b>siRNA may be effective in inhibiting SARS-associated coronavirus replication.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Replicación Viral
/
Células Vero
/
Transfección
/
Chlorocebus aethiops
/
ARN Interferente Pequeño
/
Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2003
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS