Determination of irisquinone by single sweep oscillopolarography / 药学学报

ABSTRACT

<p><b>AIM</b>To propose a polarographic method for the determination of irisquinone.</p><p><b>METHODS</b>A reduction wave of irisquinone was recorded by single sweep oscillopolarography.</p><p><b>RESULTS</b>In 8.0 x 10(-3) mol.L-1 Na2B4O7-1.6 x 10(-2) mol.L-1 KH2PO4 (pH 7.7) supporting electrolyte, a redution wave of irisquinone with peak potential -1.23 V (vs SCE) achieved high sensitivity. The 2nd-order derivative peak current of the reduction wave was proportional to irisquinone concentration in the range of 1.5 x 10(-7)-5.2 x 10(-6) mol.L-1 (gamma = 0.9992, n = 9). The detection limit was 6.0 x 10(-8) mol.L-1. Relative standard deviation (RSD) was 0.87% by performing 13 independent measurements on 2.0 x 10(-6) mol.L-1 irisquinone.</p><p><b>CONCLUSION</b>The proposed method was sensitive, simple, rapid, and can be applied to the determination of irisquinone in raw medicine and capsule.</p>