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Effects of chronic exposure to beta-amyloid-peptide25-35 on the mRNA expressions of voltage-gated outward potassium channel subunits in cultured rat hippocampal neurons / 药学学报
Acta Pharmaceutica Sinica ; (12): 598-602, 2002.
Artículo en Chino | WPRIM | ID: wpr-312073
ABSTRACT
<p><b>AIM</b>To investigate mRNA expression changes of voltage-gated outward potassium channel subtypes in cultured rat hippocampal neurons after chronic exposure to beta-amyloid-petitde25-35 (beta-AP25-35).</p><p><b>METHODS</b>mRNA expression was detected by RT-PCR, comparative expression levels were determined by imaging densitometer.</p><p><b>RESULTS</b>Delayed rectifying (Kv2.1, Kv1.5), transient outward (Kv1.4, Kv4.2) and large conductance calcium-activated (rSlo) potassium channel mRNA were expressed in cultured rat hippocampal. In the presence of beta-AP25-35 3 mumol.L-1 for 24 h, the relative expression level of Kv2.1 was significantly increased (n = 3, P < 0.05); the other subtypes were not changed obviously (n = 3, P > 0.05). The increase of Kv2.1 mRNA mainly happened between 24 and 36 h after exposure to beta-AP25-35. After exposure to beta-AP25-35 for 60 h, Kv2.1 mRNA decreased significantly (n = 3, P < 0.01).</p><p><b>CONCLUSION</b>The upregulation of Kv2.1 on transcription levels may be involved in the enhancement of delayed rectifying outward potassium (Ik) current induced by beta-AP25-35.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fragmentos de Péptidos / ARN Mensajero / Canales de Potasio / Expresión Génica / División Celular / Células Cultivadas / Péptidos beta-Amiloides / Ratas Wistar / Biología Celular / Canales de Potasio con Entrada de Voltaje Límite: Animales Idioma: Chino Revista: Acta Pharmaceutica Sinica Año: 2002 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fragmentos de Péptidos / ARN Mensajero / Canales de Potasio / Expresión Génica / División Celular / Células Cultivadas / Péptidos beta-Amiloides / Ratas Wistar / Biología Celular / Canales de Potasio con Entrada de Voltaje Límite: Animales Idioma: Chino Revista: Acta Pharmaceutica Sinica Año: 2002 Tipo del documento: Artículo