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E. coli-based production of recombinant FALL-39 / 生物医学工程学杂志
J. biomed. eng ; Sheng wu yi xue gong cheng xue za zhi;(6): 634-637, 2003.
Article en Zh | WPRIM | ID: wpr-312909
Biblioteca responsable: WPRO
ABSTRACT
This study was aimed at constructing a prokaryotic expression system to resolve the difficulties in acquiring antibacterial peptide and to meet the needs of research and drug development. Total RNA was extracted from human pulmonary gland epithelial cell line SPC-A-1, and a cDNA encoding mature FALL-39 peptide was amplified by RT-PCR. The recombinant prokaryotic expression vector pGEX-1 lambda T-FALL-39 was constructed. Using affinity chromatography, thrombin cleaving and AU-PAGE elution, we obtained the purified FALL-39. MIC, MEC, MBC analyses demonstrated that the FALL-39 had strong antibacterial activity.
Asunto(s)
Texto completo: 1 Índice: WPRIM Asunto principal: Biosíntesis de Péptidos / Péptidos / Farmacología / Proteínas Recombinantes / Escherichia coli / Vectores Genéticos / Genética Límite: Humans Idioma: Zh Revista: J. biomed. eng / Sheng wu yi xue gong cheng xue za zhi Año: 2003 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Biosíntesis de Péptidos / Péptidos / Farmacología / Proteínas Recombinantes / Escherichia coli / Vectores Genéticos / Genética Límite: Humans Idioma: Zh Revista: J. biomed. eng / Sheng wu yi xue gong cheng xue za zhi Año: 2003 Tipo del documento: Article