Effects of decitabine on biological behavior of U266 cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 944-948, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-313859
ABSTRACT
This study was aimed to explore the effects of decitabine on the biological behaviour of U266 cells in vitro so as to provide a new thinking and experiment basis, as well as new evidences for the pathogenesis of multiple myeloma. MTT and colony formation assays were used to evaluate the impact of decitabine on the ability of proliferation of U266 cells; flow cytometry was used to analyze the cell distribution in cell cycle; transwell chamber and matrigel assays were used to observe the ability of migration and invasion. The results indicated that decitabine could significantly suppress the proliferation of U266 cells in time-and dose-dependent manners. The flow cytometric analysis demonstrated that the cells in G(0)-G(1) phase significantly increased while the cells in S and G(2)/M phase decreased. The migration and matrigel invading tests showed that the number of cells moving into under chamber of transwell decreased after U266 cells treated with decitabine. It is concluded that decitabine may act as an effective drug for MM by inhibiting the proliferation, migration and invasion ability, and the specific mechanism needs to be deeply explored.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Azacitidina
/
Ciclo Celular
/
Apoptosis
/
Línea Celular Tumoral
/
Proliferación Celular
/
Mieloma Múltiple
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2011
Tipo del documento:
Artículo
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