Effect of different concentrations of bortezomib on the expression of ERK, JNK and P38 in daunorubicin-resistant K562 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 671-675, 2011.
Article
en Zh
| WPRIM
| ID: wpr-313919
Biblioteca responsable:
WPRO
ABSTRACT
The aim of this study was to investigate the effect of proteasome inhibitor bortezomib on the expression of ERK, JNK, and P38 in daunorubicin (DNR)-resistant K562 cells and its mechanism. MTT method was used to determine the drug-resistant K562 cells and the cellular toxicity of bortezomib; Western blot was used to detect the expression of protein ERK, JNK and P38 in K562 cells after treatment with 100 nmol/L DNR alone or combined with 1 nmol/L and 10 nmol/L bortezomib for 36 hours. Flow cytometry assay was used to detect the apoptosis rate in each group cells. The results indicated that the expression of ERK and P38 were significantly suppressed (p < 0.05) and the expression of JNK was significantly enhanced (p < 0.05) in the cells treated by DNR combined with bortezomib. It is concluded that bortezomib can decrease the expressions of protein ERK and P38 and enhance the expression of JNK, the bortezomib reverses the cellular drug-resistance and promote cell apoptosis through MAPK pathway.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Farmacología
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Inhibidores de Proteasas
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Pirazinas
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Ácidos Borónicos
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Resistencia a Antineoplásicos
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Células K562
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Proteínas Quinasas JNK Activadas por Mitógenos
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Proteínas Quinasas p38 Activadas por Mitógenos
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Bortezomib
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Metabolismo
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2011
Tipo del documento:
Article