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Reversal of multidrug resistance in HL-60, MSC and CD34(+) cells from umbilical cord blood by sustained intracellular acidification / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 666-670, 2011.
Artículo en Chino | WPRIM | ID: wpr-313920
ABSTRACT
The aim of this study was to investigate the effect of intracellular acidification on accumulation of rhodamine 123 (rh123) in non-mature cells with none or low expression of multidrug resistance MDR1. The expression of MDR1 mRNA was detected by real-time quantitative RT-PCR. Confocal laser microscopy was used to determine the calibration curve of intracellular acidification (pHi). MTT assay was used to detect the cytotoxicity of intracellular acidification on HL-60, MSC and CD34(+) cells from umbilical cord blood. Flow cytometry was applied to measure the influence of intracellular acidification. The results indicated that the intracellular acidification had no obvious cytotoxicity on HL-60, MSC and CD34(+) cells. The acidification resulted in the increased rhodamine 123 accumulation in HL-60, MSC and CD34(+) cells without P-gp activity. Moreover, the more primitive cells, the less accumulation of intracellular Rh123 were observed. It is concluded that the intracellular acidification can reverse the MDR of HL-60, MSC and CD34(+) cells.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Células de la Médula Ósea / Fenómenos Fisiológicos Celulares / Miembro 1 de la Subfamilia B de Casetes de Unión a ATP / Resistencia a Múltiples Medicamentos / Células HL-60 / Resistencia a Antineoplásicos / Antígenos CD34 / Biología Celular / Sangre Fetal / Células Madre Mesenquimatosas Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2011 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Células de la Médula Ósea / Fenómenos Fisiológicos Celulares / Miembro 1 de la Subfamilia B de Casetes de Unión a ATP / Resistencia a Múltiples Medicamentos / Células HL-60 / Resistencia a Antineoplásicos / Antígenos CD34 / Biología Celular / Sangre Fetal / Células Madre Mesenquimatosas Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2011 Tipo del documento: Artículo