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Early apoptosis of HL-60 cells induced by anti-CD44 McAb A3D8 inhibiting ERK1/2-upregulated Bim expression / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 656-660, 2011.
Artículo en Chino | WPRIM | ID: wpr-313922
ABSTRACT
This study was aimed to investigate the effects of anti-CD44 mAb A3D8 on proliferation and apoptosis of AML cells, to explore the mechanism of ERK1/2 and Bim in this process. Effect of the anti-CD44 mAb A3D8 on the HL-60 cell proliferation was assayed with MTT method, the change of mitochondrial transmembrane potential of HL-60 cells was analyzed by flow cytometry. The mRNA expression of Bim was determined by real-time quantitative RT-PCR. Western blot was used to detect the protein expression of p-ERK1/2. The results showed that mAb A3D8 could remarkably inhibit the proliferation capacity of the HL-60 cells in a dosage- and time-dependent ways. The mitochondrial transmembrane potential in HL-60 cells treated with A3D8 (3.0 µg/ml) was significantly decreased as compared with the control cells. Furthermore, the mRNA expression of Bim was much higher than that in controls. Expression of the p-ERK was much lower than that of the controls. It is concluded that anti-CD44 mAb A3D8 can inhibit the proliferation and induce the apoptosis of HL-60 cells, mechanism of which is enhancing the expression of Bim via inhibiting p-ERK1/2.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Regulación Leucémica de la Expresión Génica / Regulación hacia Arriba / Proteínas Proto-Oncogénicas / Apoptosis / Células HL-60 / Receptores de Hialuranos / Proteína Quinasa 1 Activada por Mitógenos / Proteína Quinasa 3 Activada por Mitógenos / Proliferación Celular Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2011 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Regulación Leucémica de la Expresión Génica / Regulación hacia Arriba / Proteínas Proto-Oncogénicas / Apoptosis / Células HL-60 / Receptores de Hialuranos / Proteína Quinasa 1 Activada por Mitógenos / Proteína Quinasa 3 Activada por Mitógenos / Proliferación Celular Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2011 Tipo del documento: Artículo