Induction of Th1 immune response against tumor by genetically engineered fusion of tumor cells and dendritic cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 61-64, 2002.
Artículo
en Chino
| WPRIM
| ID: wpr-314662
ABSTRACT
<p><b>OBJECTIVE</b>To study the antitumor activity of engineered fusion of tumor cell and dendritic cells (DC).</p><p><b>METHODS</b>J558 tumor cells were transfected with mouse IL-12 (mIL-12) gene and then fused with DCs to develop a hybrid-engineered tumor vaccine. BALB/c mice were challenged with wild-type J558 tumor cells 14 days after vaccinated with hybrid-engineered J558.</p><p><b>RESULTS</b>mIL-12 was detected at (870 +/- 60) pg.(10(5) cells)(-1).ml(-1) in the culture supernatants and the cell-fusion rate was about 30% by co-focal microscopy. In addition, the lymphocytes from popliteal nodes and groin nodes of these mice vaccinated with hybrid-engineered J558 secreted higher levels of IFN-gamma than that of other control mice, and vaccination of mice with the fusion vaccine induced more efficient tumor-specific CTL cytotoxicity against wild-type tumor cells in vitro and with efficient antitumor immunity in vivo.</p><p><b>CONCLUSION</b>It suggested that vaccination of mice with the fusion vaccine induced stronger Th1-dominant responses and this approach could perhaps be applied to clinical settings of DCs-based cancer vaccines.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células Dendríticas
/
Células Tumorales Cultivadas
/
Transfección
/
Linfocitos T Citotóxicos
/
Fusión Celular
/
Tasa de Supervivencia
/
Mortalidad
/
Interferón gamma
/
Células TH1
/
Microscopía Confocal
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Hematology
Año:
2002
Tipo del documento:
Artículo
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