Adenoviral transduction of endostatin gene prevents the liver metastasis of colorectal carcinoma in postoperation / 中华外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the prevention of colorectal cancer liver metastasis by adenoviral transduction of the endostatin gene.</p><p><b>METHODS</b>The recombinant adenovirus expressing endostatin was constructed. Its biological activities were surveyed in vitro, as determined in human umbilicus vein endothelium cell (HUVEC) proliferation inhibition, and in vivo, by reduction of liver metastasis.</p><p><b>RESULTS</b>HUVEC proliferation was obviously inhibited by the infecting supernatant of recombinant adenovirus. Persistent high serum levels of endostatin in peripheral blood, especially in the liver vein were observed. The production of liver metastasis was intervened.</p><p><b>CONCLUSIONS</b>The single injection in the vein of the recombinant adenovirus realizes the high effective and stable expression of endostatin in general body and liver, which brings about the ideal prevention of liver metastasis.</p>