Establishment of a quantitative mathematical model of Shen-deficiency syndrome based on whole-genome transcriptional profiles / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 131-134, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-315210
ABSTRACT
<p><b>OBJECTIVE</b>To establish a quantitative mathematical model of Shen-deficiency syndrome of TCM by utilizing whole-genome transcriptional profiles.</p><p><b>METHODS</b>The 4, 10, 18, 24 months old SD rats were used, 24-months aged rats intervened by Epimedium Flavonoids (EF) were adopted in the experiment. Rats' hypothalamus, pituitary, adrenal, lymphocytes, bone, liver, and kidney, and spleen were taken for determining whole-genome mRNA expression with gene chip, and a quantitative nerve network model was established by utilizing the gene expression profile of different aged rats, then the model was used to evaluate the effects of EF on Shen-deficiency syndrome.</p><p><b>RESULTS</b>Totally 199 genes showing age-dependent characteristics were screened out from the 7 kinds of tissue, most of them were neuro-endocrine immune related genes. Evaluation based on the mathematical model showed the age of hypothalamus, pituitary, adrenal, liver, kidney, bone, and spleen in the 24-months rats after EF intervention was 12.64, 10.87, 8.10, 12.70, 11.93, 13.14, and 10.13 months, respectively.</p><p><b>CONCLUSION</b>A quantitative mathematical model can be established based on the gene expression profile, it is suitable for estimating the efficacy of Shen-tonifying drugs. EF can make the gene expression of elder close to the young state, suggesting that EF has action in improving Shen-deficiency syndrome and delaying senescence.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Síndrome
/
Medicamentos Herbarios Chinos
/
Química
/
Deficiencia Yang
/
Ratas Sprague-Dawley
/
Perfilación de la Expresión Génica
/
Epimedium
/
Diagnóstico
/
Diagnóstico Diferencial
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Año:
2008
Tipo del documento:
Artículo
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