Expression of Total Vascular Endothelial Growth Factor and the Anti-angiogenic VEGF 165 b Isoform in the Vitreous of Patients with Retinopathy of Prematurity / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2505-2509, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-315306
ABSTRACT
<p><b>BACKGROUND</b>This study was to examine the expression of total vascular endothelial growth factor (VEGF) and the anti-angiogenic VEGF 165 b isoform in the vitreous body of retinopathy of prematurity (ROP) patients, and to further study the role of the VEGF splicing in the development of ROP.</p><p><b>METHODS</b>This was a prospective clinical laboratory investigation study. All patients enrolled received standard ophthalmic examination with stage 4 ROP that required vitrectomy to collect the vitreous samples. The control samples were from congenital cataract patients. The expression of total VEGF and the anti-angiogenic VEGF 165 b were measured by enzyme-linked immunosorbent assay. Results were analyzed statistically using nonparametric tests.</p><p><b>RESULTS</b>The total VEGF level was markedly elevated in ROP samples while VEGF 165 b was markedly decreased compared to control group. The relative protein expression level of VEGF 165 b isoform was significantly decreased in ROP patients which were correlated with the ischemia-induced neovascularization.</p><p><b>CONCLUSIONS</b>There was a switch of VEGF splicing from anti-angiogenic to pro-angiogenic family in ROP patients. A specific inhibitor that more selectively targets VEGF 165 and controls the VEGF splicing between pro- and anti-angiogenic families might be a more effective therapy for ROP.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cuerpo Vítreo
/
Retinopatía de la Prematuridad
/
Recien Nacido Prematuro
/
Ensayo de Inmunoadsorción Enzimática
/
Estudios Prospectivos
/
Isoformas de Proteínas
/
Factor A de Crecimiento Endotelial Vascular
/
Metabolismo
Tipo de estudio:
Estudio observacional
Límite:
Femenino
/
Humanos
/
Masculino
/
Recién Nacido
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2015
Tipo del documento:
Artículo
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