Changes of microRNA expression profiles in Vero cells induced by HSV-2 LAT overexpression / 南方医科大学学报
Journal of Southern Medical University
; (12): 1440-1444, 2012.
Article
en Zh
| WPRIM
| ID: wpr-315446
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes in the microRNA expression profile of Vero cells induced by HSV-2 LAT overexpression.</p><p><b>METHODS</b>The full-length open reading frame of HSV-2 LAT was synthesized and cloned into pRetroQ- AcGFP1-C1 vector, and the recombinant retrovirus expressing HSV-2 LAT was packaged. Using a microRNA microarray, the microRNA expression profile changes in Vero cells were analyzed after infection with the recombinant retrovirus.</p><p><b>RESULTS</b>In Vero cells infected with the recombinant retrovirus for stable HSV-2 LAT overexpression, 5 microRNAs (hsa-miR-23a*, kshv-miR-K12-3, hsa-miR-943, hsa-miR-634, and hsa-miR-1270) were up-regulated and 5 (hsa-miR-181a-2*, hsa-miR-450b-5p, hsa-miR-31, hsa-miR-24, and kshv-miR-K12-12*) were down-regulated.</p><p><b>CONCLUSION</b>The expression of HSV-2 LAT can induce changes in microRNA expression profile in Vero cells.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Proteínas Virales
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Células Vero
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Chlorocebus aethiops
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Clonación Molecular
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Herpesvirus Humano 2
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Análisis de Secuencia por Matrices de Oligonucleótidos
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Perfilación de la Expresión Génica
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MicroARNs
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Genética
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Metabolismo
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2012
Tipo del documento:
Article